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. 2021 May 19;12:671337. doi: 10.3389/fgene.2021.671337

FIGURE 4.

FIGURE 4

Circ-MFN2 directly interacted with miR-574-3p. (A) The predicted and mutated binding sites between circ-MFN2 and miR-574-3p were shown. (B,C) Dual-luciferase reporter assay was used to verify the interaction between circ-MFN2 and miR-574-3p. (D) The expression of miR-574-3p in CRC tumor tissues and non-cancer tissues was measured using qRT-PCR. (E) QRT-PCR was used to detect the miR-574-3p expression in CRC cell lines (SW620 and SW480) and NCM460 cells. (F) Pearson correlation analysis was used to assess the correlation between circ-MFN2 and miR-574-3p. (G) The transfection efficiencies of si-circ-MFN2 and pcDNA circ-MFN2 overexpression plasmid were confirmed using qRT-PCR. (H) QRT-PCR was employed to detect the expression of miR-574-3p to assess the effect of circ-MFN2 on miR-574-3p expression. *P < 0.05.