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. 2021 May 26;24(1):538. doi: 10.3892/mmr.2021.12177

Figure 2.

Figure 2.

Effect of miR-483-5p on TGF-β1-induced EMT. (A) Relative expression of miR-483-5p in A549 cells after 24 h transfection with miR-483-5p mimic was detected via RT-qPCR. **P<0.01 vs. mimic NC. Following treatment with TGF-β1 for 48 h, relative of expression of (B) E-cadherin, (C) vimentin and (D) α-SMA were determined by RT-qPCR and (E and F) western blotting. A549 cells were transfected with miR-483-5p inhibitor for 24 h, and then treated with TGF-β1 for 48 h. *P<0.05; **P<0.01. (G) Relative expression of miR-483-5p in A549 cells following transfection with inhibitor. **P<0.01 vs. inhibitor NC. The relative of expression of (H) E-cadherin, (I) vimentin and (J) α-SMA were determined by RT-qPCR and (K and L) western blotting. Data are presented as the mean ± SD (n=3). *P<0.05; **P<0.01. NS, not significant; EMT, epithelial-mesenchymal transition; TGF-β1, transforming growth factor-β1; α-SMA, α-smooth muscle actin; miR, microRNA; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control.