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. 2021 May 26;24(1):538. doi: 10.3892/mmr.2021.12177

Figure 4.

Figure 4.

miR-483-5p inhibitor inhibits TGF-β1-induced EMT by targeting RhoGDI1 via the Rac1/PI3K/AKT signaling pathway. The relative expression of RhoGDI1 in A549 cells infected with lentivirus carrying the interference sequence of RhoGDI1 was detected by (A) RT-qPCR and (B and C) western blotting. **P<0.01 vs. shRNA-NC. A549 cells or A549 cells with shRNA-RhoGDI1 silencing were transfected with miR-483-5p inhibitor for 24 h, and then treated with TGF-β1 for 48 h. The relative of expression of (D) E-cadherin, (E) vimentin and (F) α-SMA were determined by RT-qPCR and (G and H) western blotting. (I) Expression of (J) Rac1-GTP, Rac1, (K) p-PI3K, PI3K, (L) p-AKT and AKT were determined by western blot analysis. Data are presented as the mean ± SD (n=3). *P<0.05; **P<0.01. NS, not significant. 1=Ctrl, 2=TGF-β1 group, 3=TGF-β1+ miR-483-5p inhibitor+ shRNA-NC group, 4=TGF-β1+ miR-483-5p inhibitor + shRNA-RhoGDI1 group. EMT, epithelial-mesenchymal transition; RhoGDI1, Rho GDP dissociation inhibitor 1; TGF-β1, transforming growth factor-β1; α-SMA, α-smooth muscle actin; miR, microRNA; Rac1, Rac family small GTPase 1; RT-q, reverse transcription-quantitative; p-, phosphorylated; sh, short hairpin; NC, negative control; Ctrl, control.