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. 2021 May 19;12:663061. doi: 10.3389/fimmu.2021.663061

Figure 8.

Figure 8

Dopamine increases NT CCR5 in C06 human microglia. (A) Change in CCR5 expression relative to the mean of vehicle-treated (diH2O) C06 cells demonstrates that dopamine increases NT but not ECL2 CCR5, similar to what we found in hMDM (N=5) (ECL2 CCR5, Paired t-test, n=5, Dopamine, p=0.1818, t=1.614, df=4; NT CCR5, Paired t-test, n=5, Dopamine, **p=0.0043, t=5.837, df=4). C06 cells were also treated with dopamine (10-6 M) for 1 hour and probed for pan NT CCR5. A representative blot is shown in (B). (C) Pooled data showing fold change in C06 cells from 4 passages relative to the vehicle condition is shown with CCR5 normalized to total protein stain. Although this did not reach significance (Paired t-test, n=4, Dopamine, p=0.2182, t=1.553, df=3), three out of four passages show an increase in NT CCR5 with dopamine relative to vehicle.