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. 2021 May 19;12:687673. doi: 10.3389/fimmu.2021.687673

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Copyright © 2021 Phillips, Schürch, Khodadoust, Kim, Nolan and Jiang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Visualizing immune cell-types by CODEX. (A, B) H&E stainings for two different CTCL patients and corresponding Voronoi diagrams from 6 select regions; see Figure 2B for full Voronoi diagram. (C) Major immune cell-types separated by lineage and shown as: 1) a zoomed-in region from one the six selected Voronoi diagrams, color-coded as in (A, B) (top panel), 2) a CODEX fluorescent image with Hoechst nuclear stain (blue) and the marker of interest (white) (middle panel), and 3) a simulated brightfield IHC image for the marker of interest (bottom panel). Scale bars: (A, B) 100 µm, (C) 10 µm.