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. 2021 May 19;12:623610. doi: 10.3389/fimmu.2021.623610

Figure 1.

Figure 1

Supernatant of CAR-T/Nalm6 co-culture induced endothelial activation. (A) The cytokine release profile in the supernatant of HUVEC incubated with sCAR-T was determined by Luminex. The Y axis represented Target to Effector ratio. (B) The mRNA levels of endothelial activation-associated markers were determined by RT-PCR. GAPDH was taken as the housekeeping gene and data was expressed as fold changes relative to control. n = 3. (C) The protein expression of E-selectin, VCAM1, and ICAM1 in HUVEC induced by sCAR-T incubation was determined by flow cytometry. (D) The protein level of tissue factor (TF) in HUVEC induced by sCAR-T incubation was analyzed by western blot. (E) The concentration of Ang2 secreted by HUVEC was assessed by ELISA. n = 3. (F) The permeability of endothelial monolayer was determined by Evans blue/BSA assay (EBA). n = 3. (G) The protein levels of indicated proteins were determined by western blot. * represents p < 0.05, ** represents p < 0.01, and *** represents p < 0.001. ns represents not significant. All data were representative of at least three independent experiments.