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. 2021 May 19;9:652939. doi: 10.3389/fcell.2021.652939

FIGURE 5.

FIGURE 5

PTEN was the target gene of miR-221/222. (A) The mature miR-221/222 sequence in different species. (B) Venn diagram showed the target genes of miR-221/222 obtained from different databases. (C) The alignment result of PTEN 3′UTR sequence from different species. Red represented the complete complementary sequence of the PTEN 3′UTR. (D) The complementary pairing of the target gene PTEN 3′UTR and miR-221/222. (E) qRT-PCR result confirmed PTEN mRNA level was negatively correlated with miR-221/222 level. (F) WB result confirmed PTEN protein level was negatively correlated with miR-221/222 level. (G) The interaction of miR-221/222 and PTEN in RNA-induced silencing complexes through RNA immunoprecipitation. (H) Luciferase reporters were transfected into HEK-293T cells with either miR-221/222 NC or miR-221/222 mimics, respectively. Luciferase value was measured 24 h after transfection. The results were presented as mean ± SEM of triplicate independent experiments for each group. Bars with different letters indicate they are significantly different (p < 0.05); **p < 0.01.