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. 2021 May 19;9:652939. doi: 10.3389/fcell.2021.652939

FIGURE 8.

FIGURE 8

MiR-221/222 protects intestinal epithelial cells against Don-induced apoptosis and proliferation inhibition by targeting PTEN. (A) Representative images of the caspase-3 immunofluorescent staining for apoptotic intestinal epithelial cells. Cleaved caspase-3: red, a molecular marker of apoptosis; DAPI: blue, cell nucleus; Merge: the apoptotic cells. (B) The quantitative result of (A). (C) Representative images of the EdU staining for proliferating intestinal epithelial cells are shown. The cell nucleus was stained with DAPI, blue. Proliferating intestinal epithelial cells were labeled with EdU fluorescent dye (red). (D) The quantitative result of (C). Our results were presented as mean ± SEM of triplicate independent experiments for each group. Bars with different letters indicate they are significantly different (p < 0.05). In (A), magnification = × 100; the scale bar on the photomicrographs represents 100 μm. In (B), magnification = × 100; the scale bar on the photomicrographs represents 100 μm. Don, IPEC-J2 cells were exposed to 1.6 μg/ml Don for 12 h. Don + miR-221/222, IPEC-J2 cells were transfected with 80 nM miR-221/222 mimics for 24 h, then exposed to 1.6 μg/ml Don for 12 h. Don + miR-221/222 + PTEN, IPEC-J2 cells were transfected with 80 nM miR-221/222 mimics and 1.5 μg/ml pcDNA3.1-PTEN for 24 h, then exposed to 1.6 μg/ml Don for 12 h.