Skip to main content
. 2021 May 19;9:652939. doi: 10.3389/fcell.2021.652939

FIGURE 9.

FIGURE 9

MiR-221/222 attenuated Don-induced intestinal mucosa damage by targeting PTEN. (A) qPCR results indicated that miR-221/222 was specifically overexpressed in intestinal epithelial cells by adenovirus. (B) Morphological observation of small intestinal villi by H&E staining. (C) Representative images of the PTEN immunofluorescent staining for apoptotic intestinal epithelial cells. PTEN: red, the target gene of miR-221/222; DAPI: blue, cell nucleus; Merge: the apoptotic cells. (D) Western blot results showed Don-induced apoptosis in intestinal epithelial cells relied on PTEN. (E) The relative protein levels obtained by WB band gray scanning. (F) qPCR results indicated that miR-221/222 attenuated Don-induced intestinal mucosa damage by targeting PTEN. Our results were presented as mean ± SEM of triplicate independent experiments for each group. Bars with different letters indicate they are significantly different (p < 0.05). In (B), magnification = × 40; the scale bar on the photomicrographs represents 500 μm. In (C), magnification = × 400; the scale bar on the photomicrographs represents 100 μm. Don, gavage mice with Don (2.5 mg/kg body weight) and injected adenovirus–CDX2 promoter–miR-221/222 negative control. Don + miR-221/222, gavage mice with Don (2.5 mg/kg body weight) and injected adenovirus–CDX2 promoter–miR-221/222.