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. 2021 May 19;9:652939. doi: 10.3389/fcell.2021.652939

TABLE 2.

The details of antibodies used in this study.

Primary antibody Clone Company Catalog no. Dilution
PTEN Monoclonal CST #9188 1:2,000
P21 Monoclonal CST #2947 1:1,000
AKT Monoclonal CST # 9272S 1:2,000
p-AKT Monoclonal CST # 4058S 1:2,000
Bcl2 Monoclonal CST #15071 1:1,000
BAX Monoclonal CST #2774 1:2,000
Caspase-3 Polyclonal CST #9662 1:1,000
Cleaved caspase-3 Polyclonal CST #9662 1:1,000
β-actin Monoclonal Bioworld BS6007M 1:5,000

Secondary antibody Conjugate used Company Catalog no. Dilution

Goat anti-rabbit IgG HRP Bioss bs-0295G 1:5,000
Goat anti-rabbit IgG (H + L) HRP Bioworld BS13278 1:50,000
Goat anti-mouse IgG (H + L) HRP Bioworld BS12478 1:50,000
Goat anti-mouse IgG (H + L) Cy3 Bioworld BS10006 1:200
Goat anti-rabbit IgG Alexa Fluor 647 Bioss bs-0295G-AF647 1:200