TABLE 2.
Primary antibody | Clone | Company | Catalog no. | Dilution |
PTEN | Monoclonal | CST | #9188 | 1:2,000 |
P21 | Monoclonal | CST | #2947 | 1:1,000 |
AKT | Monoclonal | CST | # 9272S | 1:2,000 |
p-AKT | Monoclonal | CST | # 4058S | 1:2,000 |
Bcl2 | Monoclonal | CST | #15071 | 1:1,000 |
BAX | Monoclonal | CST | #2774 | 1:2,000 |
Caspase-3 | Polyclonal | CST | #9662 | 1:1,000 |
Cleaved caspase-3 | Polyclonal | CST | #9662 | 1:1,000 |
β-actin | Monoclonal | Bioworld | BS6007M | 1:5,000 |
Secondary antibody | Conjugate used | Company | Catalog no. | Dilution |
Goat anti-rabbit IgG | HRP | Bioss | bs-0295G | 1:5,000 |
Goat anti-rabbit IgG (H + L) | HRP | Bioworld | BS13278 | 1:50,000 |
Goat anti-mouse IgG (H + L) | HRP | Bioworld | BS12478 | 1:50,000 |
Goat anti-mouse IgG (H + L) | Cy3 | Bioworld | BS10006 | 1:200 |
Goat anti-rabbit IgG | Alexa Fluor 647 | Bioss | bs-0295G-AF647 | 1:200 |