. 2021 May 24;44:102014. doi: 10.1016/j.redox.2021.102014

Table 2.

Actin modification sites identified in SIN-1 treated and untreated cardiac cells. Modification sites are numbered for the mature protein without propeptide as provided by UniProtKB. *indicates modifications via Schiff base formation. ^Δcytoplasmic actin isoforms: P60711, P63259. ⁺actin isoforms: P68035, P62738, P63269, P68136.

AA	#_modified	#_{modifications}	modified residues	modifications
Arg	2	2	R256^w, R290ⁱ	^wGlutamic semialdehyde, ⁱGlyoxal*
Cys	4	6	C217^bchsy, C257^chy, ^ΔC272^hy, C285^bghs	^bDioxidation, ^cTrioxidation, ^yGlutathionylation, ^gGlyoxal, ^hHexenal, ^sOxo-pentanal,
His	9	4	H40^ahq, H73^aj, H87^ah, H88^h, H101^ah, H161^ahq, H173^ah, H275^a, H371^h	^aOxidation, ^hHexenal, ^jHHE, ^qPentenal
Lys	12	13	K50^defhoq, K61^dfho, K68^eflm, K84^efmv, K113^dfht, K118^f, K191^fhq, K291^fghms, K315^fh, K326^dfhjt, K328^df, K373^f	^dAcetylation, ^vCrotonylation, ^eDimethylation, ^fFormylation, ^gGlyoxal, ^hHexenal, ^jHHE, ^lHNE, ^oMalonylation, ^mMethylation, ^tOxo-hexanoic acid*/Oxo-hexenal, ^sOxo-pentanal, ^qPentenal
Met	14	2	M44^ab, M47^ab, M82^ab, M132^a, ^ΔM153^ab, ⁺M176^a, M190^ab, M227^a, M269^a, M283^a, M305^ab, M313^ab, M325^ab, M355^a	^aOxidation, ^bDioxidation
Phe	3	1	F223^a, ^ΔF279^a, F352^a	^aOxidation
Pro	10	2	P38^a, P70^az, P98^a, P102^a, P164^a, P172^a, P243^a, P264^z, P307^a, P322^a	^aOxidation, ^zPyroglutamic acid
Ser	1	1	S52^p	^pPhosphorylation
Thr	4	1	T148/149¹, T160¹, T229¹, T303¹, T303/304¹	¹2-amino-3-ketobutyric acid
Trp	4	4	W79^ab3, W86^abc3, W340^a, W356^ab	^aOxidation, ^bDioxidation, ^cTrioxidation, ³Kynurenine
Tyr	12	4	Y53^a, Y69^a5, Y91^a5, Y166^a, Y169^a6, Y188^a, Y198^a5, Y240^a56, ⁺Y279^a, Y294^a5, Y306^a, Y337^a	^aOxidation, ^bDioxidation, ⁵Dopaquinone, ⁶Nitration