Figure 4.

Expression profile of lincRNAs: (a) Average expression levels of lincRNAs and protein coding transcripts. (b) Heatmap of lincRNA expression profiles across development, where lincRNAs were clustered based on the stage of maximum expression. (c) Visualization of the number of differentially expressed lincRNAs between each pair of stages. The size of the circles represents the number of differentially expressed lincRNAs. Blue and red represent the up- and downregulated lincRNAs; lincRNAs with |log₂^{fold change}| >1 and FDR <0.01 were considered differentially expressed lincRNAs. (d) Number of significantly up- and downregulated genes between stage transitions. (e) Validation of differentially expressed lincRNAs using qRT-PCR. The line graph represents the qRT-PCR data and the Heatmap in the bottom represents the RNA-Seq data. Abbreviations: FDR, false discovery rate; lincRNAs, Long noncoding RNAs. (Color version of figure is available online.)