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Effects of PAMK on the relative mRNA expression of CD25, novel_mir2, CTLA4 and CD28 signal pathway of lymphocytes in thymus treated with different transfection reagents. Relative mRNA expression of (A) CD25; (B) novel_mir2; (C) CTLA4. (D) CD28; (E) PI3K; (F) VAV2; (G)JNK; (H)AP-1. Data are expressed as the means ± SD, n = 9 *P < 0.05, **P < 0.01, ***P < 0.001, compared with NC mimic group; #P < 0.05, ##P < 0.01, ###P < 0.001, compared with novel_mir2 inhibitor group.
