Figure 4.

p97 inhibition and depletion negatively regulate the anti-resection factors 53BP1 and REV7, causing a delay in DNA double-strand break (DSB) repair

(A–C) Representative images (A) and quantification of 53BP1 (B) and γH2AX (C) foci kinetics over 24 h in p97-depleted T24 cells after 2 Gy IR. Scale bars, 5 μm; n = 3; Mann-Whitney test. NS, not significant; ^∗∗∗∗p < 0.0001.

(D and E) Representative images (D) and quantification of REV7 (E) foci kinetics in p97-depleted T24 cells over 24 h after 2 Gy IR. Scale bars, 5 μm. Data are presented as boxplot with interquartile ranges shown, and values beyond the 5th and 95th percentiles shown as individual points; n = 3; Mann-Whitney test. NS, not significant; ^∗∗∗∗p < 0.0001.

(F) Western blot demonstrating the efficiency of siRNA p97 knockdown.

See also Figure S5.