. 2021 May 19;15:665386. doi: 10.3389/fncir.2021.665386

TABLE 1.

TH and VGluT2 coexpression in midbrain DA neurons.

				TH⁺VGLUT2⁺/Total TH⁺%
Age	Species	Genotype	Method	Midbrain (Total)	Medial-only				Lateral-only		Citation
E11	Mouse	WT	ISH		(>E14)						Dumas and Wallén-Mackenzie, 2019
E14	Mouse	WT	ISH		(<E11)
E14	Mouse	TH^EGFP	sc RT-PCR	7							Fortin et al., 2012
E16				47
E18				33
E15, 16	Rat	WT	ISH	(High)							Dal Bo et al., 2008
E18, 21				(Low)
P0	Mouse	TH^EGFP	dissociation, sc RT-PCR	25							Mendez et al., 2008b
P0	Mouse	VGluT2^EGFP bacterial artificial chromosome	IHC (TH, EGFP)	2
P0-2	Mouse	TH-EGFP	sc RT-PCR	22	36				13		Fortin et al., 2012
P5	Rat	WT	ISH		3				<1		Dal Bo et al., 2008
P10	Mouse	VGluT2^EGFP bacterial artificial chromosome	IHC (TH, EGFP)	1							Mendez et al., 2008b
P10	Rat	WT	ISH		2				<1		Dal Bo et al., 2008
P14	Mouse	TH^EGFP	sc RT-PCR	14							Fortin et al., 2012
P14	Mouse	TH^EGFP	sc RT-PCR		18				14		Mendez et al., 2008b
P15	Rat	WT	ISH		2				<1		Dal Bo et al., 2008
P35	Mouse	TH^EGFP	sc RT-PCR	30							Fortin et al., 2012
P45	Mouse	TH^EGFP	dissociation, sc RT-PCR	14							Mendez et al., 2008b
P45	Mouse	VGluT2^EGFP bacterial artificial chromosome	IHC (TH, EGFP)	<1
6–24 weeks	Mouse	WT	ISH (RNA Scope)		56		37				Yan et al., 2018
					Medial VTA		Lateral VTA
P70	Mouse	TH^EGFP	sc RT-PCR	47	78				25		Fortin et al., 2012
P90	Rat	WT	ISH		2				<1		Bérubé-Carrière et al., 2009
8–12 weeks	Mouse	DAT^IRES–Cre; VGluT2^flox/+	ISH		15				20		Shen et al., 2018
Adult	Rat	WT	ISH		<1						Yamaguchi et al., 2007
Adult	Rat	WT	ISH		PBP	3	IF	22			Kawano et al., 2006
					PN	5	CLi	22
					A10	19	RLi	53
Adult	Rat	WT	TH-IHC, VGluT2-ISH	Medial PBP		60	IF	10			Li et al., 2013
				medial PN		50	RLi	60
			Laser micro-dissection, sc RT-PCR		medial PBP			42
							RLi	42
							IF	57
Adult	Marmoset	WT	TH-IHC, VGluT2-ISH	PBP		23	IF	2	Ventral SNc	<1	Root et al., 2016
				PN		2	CLi	2	Dorsal SNc	<1
				Caudal VTA		5	RLi	3	Medial SNc	<1
				Rostral VTA		6	PIF	4	Lateral SNc	< 1
Adult (55 years)	Human	WT	TH-IHC, VGluT2-ISH		PBP	17	VTA	10	Ventral SNc	2
					PN	2	RLi	10	Dorsal SNc	3
					VTA subdivision			10	Medial SNc	3
									Lateral SNc	<1