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. 2021 Jun 1;2021:10.17912/micropub.biology.000403. doi: 10.17912/micropub.biology.000403

Figure 1. ASI1 and ASI3 confer resistance to hygromycin B.

Figure 1. ASI1 and ASI3 confer resistance to hygromycin B

(A-C) Sixfold serial dilutions of yeast of the indicated genotypes were spotted onto agar plates containing rich growth medium (No Drug) or indicated concentrations of hygromycin B. Plates were incubated at 30°C and imaged after 1-3 days. Experiments were performed in triplicate. (C)asi2Δ (YKO)” is VJY852 and was obtained from the Yeast Knockout Collection (Tong et al., 2001). “asi2Δ (new clone 1)” and “asi2Δ (new clone 2)” are VJY969 and VJY970, respectively, and were generated for this study.