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. Author manuscript; available in PMC: 2022 Jun 15.
Published in final edited form as: Bioorg Med Chem. 2021 May 1;40:116183. doi: 10.1016/j.bmc.2021.116183

Figure 1: Equilibrium dialyses of IMP-1 with inhibitors.

Figure 1:

IMP-1 (8 μM) was incubated with varying concentrations of inhibitor for an hour on ice, and then then the enzyme/inhibitor mixture was dialyzed for 8 hours against 50 mM HEPES, pH 7.5. The metal content of the resulting samples was determined by using atomic absorption spectrometry.9 The average standard deviation of the Zn content determined by AA is 0.09 μM, as previously reported.9