Figure 4. SARS-CoV-2 RT-LAMP is highly sensitive, robust, and precise.

( A–B) NIBSC SARS-CoV-2 standard was serially diluted and the indicated number of copies in 4.5 µL was assessed by N gene RT-LAMP. Amplification curves shown with the limit of detection (L.O.D.) determined by the presence or absence of amplification following the depicted dilution in ( A) or via colorimetric read-out ( B). ( C) RNA extracted from laboratory grown SARS-CoV-2 was serially diluted 10-fold and assessed by RT-LAMP in the presence or absence of 1% Triton-X 100. ( D) A COVID-19 positive patient sample was RNA extracted independently 5 times through the CCC pipeline and subjected to 5 independent N gene RT-LAMP reactions. Assay precision for N gene and 18S was determined by calculating the coefficient of variation between Ct values observed (CV).