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. 2021 May 24;22(2):793. doi: 10.3892/etm.2021.10225

Figure 3.

Figure 3

HMGA1 is a target of miR-296-5p in CRC cells. (A) Schematic representation of potential binding sites of miR-296-5p in the 3'-UTR of HMGA1. Dual luciferase activity of CRC cells following transfection with miR-296-5p mimics or control miRNA in (B) HCT116 and (C) HT-29 cells. The (D) mRNA and (E) protein expression of HMGA1 in HCT116 and HT-29 cells, following transfection with miR-296-5p. (F) Transfection with miR-296-5p mimic significantly decreased its expression in CRC cells. The (G) mRNA and (H) protein expression level of HMGA1 in CRC cells, following the overexpression of miR-296-5p by the antagomir. ***P<0.001 vs. respective miR-NC group. HMGA1, high mobility group AT-hook 1; miR/miRNA, microRNA; CRC, colorectal cancer; UTR, untranslated region; NC, negative control; WT, wild-type.