Figure 6.

Effect of puerarin on the NF-κB pathway. (A) Western blot analysis for the protein expression levels of p-NF-κB p65 and total NF-κB p65 in lung tissues. Results were quantified as the ratio of phosphorylated to total protein. (B) Western blot analysis for the protein expression levels of IκB-α in lung tissues. Results were quantified as the ratio of IκB-α signal to GAPDH. Data are presented as mean ± standard error of the mean from three independent experiments (n=10 per group). ^*P<0.05, ^**P<0.01 vs. Con group; ^#P<0.05 vs. ALI group. p-, phosphorylated; IκB-α, NF-κB inhibitor α; Con, normal control group; ALI, smoke inhalation group; ALI + Pue, puerarin treatment plus smoke inhalation group.