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. Author manuscript; available in PMC: 2021 Jun 2.
Published in final edited form as: J Am Chem Soc. 2019 Aug 1;141(32):12582–12591. doi: 10.1021/jacs.9b03250

Figure 3.

Figure 3.

Characterization of quadruplex nanostructures. a, left) Non-denaturing PAGE (120 V at RT). Control lane 3 (and 6) is double stranded DNA ladder and lane 4 is single stranded PL-polyT. All G-rich sequences were first annealed to form G-quadruplex assembly: PL-DNA 1 (lane 1), PL-DNA 2 (lane 2), parent DNA 2 (G4T2G4) (lane 5), Porph-DNA 1 (lane 7), and Porph-DNA 2 (lane 8).. (a, right) Non-denaturing PAGE (60 V at 4 °C). Control lane 9 is double stranded DNA ladder. The G-rich sequences were first annealed to form G-quadruplex assembly: PL-DNA 1 (lane 10), and PL-DNA 2 (lane 11). b) AFM image of G-wires of parent DNA 2 (not conjugated with porphyrin). c) AFM image of annealed PL-DNA 2. d) Cry-oTEM image of annealed PL-DNA 2. e) Confocal microscopy image of G-wires of PL-DNA 2. Exciting laser was 561 nm.