Figure 1: 4E-BP1 Phosphorylation in WT N2aC.
C:Y was measured in WT N2aC transfected with TORCAR alone. Cells were incubated in (A) complete media, (B) AAF media, (C) complete media with rapamycin, or (D) in complete media with torin1 after a 10 minute baseline in complete media for each condition. A non-significant 4.2% increase in C:Y is observed over the 60 minute recording period in cells incubated in complete media while there was a 12.2% decrease in C:Y in WT cells incubated in AAF media (E, p<0.05). WT N2aC incubated with rapamycin (C) showed no change in C:Y, while exposure to torin1 (D, p<0.05) led to a 7.9% decrease in C:Y (E). Only co-transfected cell were used for live-cell imaging experiments. Error bars in (E), standard error of the mean.*p<0.05.
