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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Exp Neurol. 2020 Aug 8;334:113432. doi: 10.1016/j.expneurol.2020.113432

Figure 3: Addition of rapamycin to AAF media does not enhance the inhibitory effect of amino acid free conditions.

Figure 3:

After a 10 minute baseline in complete media WT, scramble, Tsc2 KO, Depdc5 KO cells transfected with TORCAR were incubated in complete media containing rapamycin (A-D, 150 nM, mins 10–30, solid bars in E) followed by incubation in AAF media containing rapamycin (150 nM, mins 30–60, striped bars in E). During incubation in complete media containing rapamycin, an average increase in C:Y was observed across each experimental group (E, not significant). After incubation in AAF media containing rapamycin, a decrease in C:Y ratio was observed in WT (7.9%; A, E; p<0.05), scramble (B,E; 4.7%; p<0.05), and Tsc2 KO (C,E; 8.1%; p<0.05). No statistically significant changes were observed in Depdc5 KO N2aC (E). *p<0.05.