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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Exp Neurol. 2020 Aug 8;334:113432. doi: 10.1016/j.expneurol.2020.113432

Figure 6: AAF conditions alter sub-cellular localization and soma size in Tsc2 KO N2aC.

Figure 6:

A) Correlation analysis (R) of fluorescence intensity of mTOR and LAMP2 (see Fig. 5) reveals high correlation between mTOR and the lysosomal membrane with R values of 0.87 (WT), 0.90 (Scram), 0.87 (Tsc2), and 0.92 (Depdc5). A statistically significant decrease in colocalization between mTOR and the lysosomal membrane was observed in WT (0.76), scramble (0.78) and Tsc2 KO (0.71) cells after incubation in AAF media (60 min incubation for each group, n = 10 cells per group, p<0.05). Depdc5 KO cells display an enhanced colocalization in complete media (R= 0.92) vs. WT, scramble and Tsc2 KO cells. Depdc5 KO cells displayed a nonsignificant decrease in (R) after AAF media incubation media (R= 0.88; n =10 cells per group). Incubation in AAF media results in decreased phosphorylated S6 levels in Tsc2 KO, scramble and WT N2aC but not Depdc5 KO N2aC (densitometry in Figure S7).