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Mast cell proteases alter the expression of bronchial epithelial barrier proteins. HBECs were stimulated with tryptase or chymase and E-CDH gene expression (a and c) and protein expression (e) as well as ZO-1 gene expression (b and d) and protein expression (e) was investigated. Representative western blot protein expression (f). Data are presented as mean ± SEM, n = 4–6 from 6 independent experiments. *P < 0.05, **P < 0.01compared to respective control
