Figure 6.

Evaluation of CD133, CD44, and ABCB1 positive cells following A549 treatment with menadione and cisplatin/gemcitabine. A549 cells were treated with a combination of cisplatin (0.25 µM)/gemcitabine (5 µM) as well as 16 µM menadione for 7 days. Cells were analyzed after incubation with PE anti-Human CD133 FITC anti-Human CD44 and PE anti-Human ABCB1 antibodies with flow cytometry. The cell population represented in the right low quadrants are considered positive live cells for each marker