Figure 6.

(A) MDR-1 and MRP1 expression in healthy, new case AML and relapsed AML-cells and correspond EVs. We first analyzed the normalized expression of MRD1 and MRP1 in cellular and vesicular compartment of all subjects. The resulting data showed that the expression of these genes were lower in EVs as compared with AML parent cells. In new cases and relapsed patients, the fold changes of both genes in EVs were increased. (B) The expression of MRD1 and MRP1in U937 cell treated with new cases and relapsed EVs were significantly increased. Data are mean ± SE of three independent experiments. (C) U937 cells was treated with actinomycin D (1 mg/mL) prior to EV incubation. After 24 h of EV integration, we did not observe any significant change in MDR-1 and MRP1 expression, indicating that the increase of these genes was not due to RNA transfer. Statistical significance were defined at ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001compared to corresponding control