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. 2021 Jun 1;11:102. doi: 10.1186/s13578-021-00612-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Localization of PRAMEL1 in the acrosome (AC) and manchette (Man). The image in A shows a step 9 spermatid. Clusters of PRAMEL1 gold particles were detected in the manchette (boxed region in A) and the enlarged image in B. A low density PRAMEL1 labeling was seen in the acroplaxome (C) (the boxed region in A). In steps 10–12 spermatids, clusters of gold particles were seen in the nucleus (N) and acrosome region (D, E), but a very few gold particles were observed in the acrosome granule (AG) or acrosome matrix (AM) located in the front head (D, E). In step 16 elongated spermatid, large clusters of gold particles were observed in nucleus and acrosome (F). Some of the clusters were in the nuclear membrane adjacent to acrosome. M: mitochondria; PNR: perinuclear ring; Unlabeled scale bar = 0.5 µm