Fig. 2.

The effect of miR-200b on macrophages polarization. miR-200b mimic and mimic NC were transfected into M0 macrophages. At 24 hours after transfection, a qRT-PCR was performed to examine the expression of miR-200b in the cells. b and c Immunofluorescence and flow cytometry were utilized to detect the number of CD86-positive cells and CD206-positive cells. d The percentages of CD86-positive and CD206-positive macrophages were analyzed in accordance with the results of flow cytometry. e and f The levels of iNOS mRNA and Arg-1 mRNA were measured by qRT-PCR. g and h The concentrations of IL-1β and CCL17 in cell supernatant of macrophages were measured by ELISA assay. ^#P < 0.05 contrasted with mimic NC