Fig. 4.

miR-200b regulated macrophage polarization via targeting KLF6. a and b The binding sites between miR-200b and KLF6 3’-UTR was predicted according to starBase v2.0 database, and the interaction of the two molecules was ensured by luciferase reporter assay. c and e M0 macrophage was transfected with miR-200b mimics and infected with the lentivirus expressing KLF6, and then the levels of KLF6 mRNA and protein in the cells were measured using qRT-PCR and western blot, respectively. ^#P < 0.05 compared with mimic NC. ^&P < 0.05 contrasted with inhibitor NC. f-h The percentages of M1 macrophage and M2 macrophage were determined by using flow cytometry. i and j qRT-PCR was carried out to detect the expression of iNOS and Arg-1 mRNAs. k and l ELISA assay was used to measure the concentration of IL-1β and CCL17 in the cell supernatant. ^#P < 0.05 compared with mimic NC. ^&P < 0.05 vs. inhibitor NC or LV-NC