Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-HSF1 monoclonal antibody [EP1710Y] | Abcam | Cat# ab52757, RRID: AB_880518 |
| Rabbit anti-LDH monoclonal antibody [EP1563Y] | Abcam | Cat# 1980-1, RRID: AB_991755 |
| Mouse anti-AMPKα1/2 monoclonal antibody | Bio-Rad | Cat# MCA2673GA; RRID: AB_1604623 |
| Donkey Anti-Mouse IgG (H+L) Highly Cross-Adsorbed Antibody, CF594 Conjugated | Biotium | Cat# 20115, RRID: AB_10559815 |
| Donkey Anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Antibody, CF594 Conjugated | Biotium | Cat# 20152, RRID: AB_10563030 |
| Rabbit anti-AMPK (23A3) monoclonal Antibody | Cell Signaling Technology | Cat# 2603, RRID: AB_490795 |
| Rabbit anti-phospho-Raptor (Ser792) polyclonal antibody | Cell Signaling Technology | Cat# 2083, RRID: AB_2249475 |
| Mouse anti Lamin A/C (4C11) monoclonal antibody | Cell Signaling Technology | Cat# 4777, RRID: AB_10545756 |
| Rabbit anti-LKB1 (D60C5) monoclonal antibody | Cell Signaling Technology | Cat# 3047, RRID: AB_2198327 |
| Mouse anti-DYKDDDDK (FLAG) Tag (9A3) monoclonal antibody | Cell Signaling Technology | Cat# 8146, RRID: AB_10950495 |
| Rabbit anti-phospho-SREBP-1c (Ser372) polyclonal antibody | Cell Signaling Technology | Cat# 9874, RRID: AB_10949508 |
| Rabbit anti-PP2A C Subunit polyclonal antibody | Cell Signaling Technology | Cat# 2038, RRID: AB_2169495 |
| Rabbit anti-phospho-AMPKα, (Thr172) (40H9) monoclonal antibody | Cell Signaling Technology | Cat# 2535, RRID: AB_331250 |
| Rabbit anti-DYKDDDDK (FLAG) Tag (D6W5B) monoclonal antibody | Cell Signaling Technology | Cat# 14793, RRID: AB_2572291 |
| Rabbit anti-GST (91G1) monoclonal antibody | Cell Signaling Technology | Cat# 2625, RRID: AB_490796 |
| Rabbit anti-phospho-Acetyl-CoA Carboxylase (Ser79) polyclonal antibody | Cell Signaling Technology | Cat# 3661, RRID: AB_330337 |
| Rabbit anti-PI3 Kinase p110α (C73F8) monoclonal antibody | Cell Signaling Technology | Cat# 4249, RRID: AB_2165248 |
| Rabbit anti-Raptor (24C12) monoclonal antibody | Cell Signaling Technology | Cat# 2280, RRID: AB_561245 |
| Rabbit anti-Acetyl-CoA Carboxylase (C83B10) monoclonal antibody | Cell Signaling Technology | Cat# 3676, RRID: AB_2219397 |
| Mouse anti-Phosphoserine / threonine monoclonal antibody | ECM Biosciences | Cat# PM3801, RRID: AB_1944434 |
| Rabbit anti-AMPKα1 monoclonal antibody | GeneTex | Cat# GTX61229; RRID: AB_10620245 |
| DYKDDDDK (FLAG) Tag antibody-HRP | GenScript | Cat# A01428, RRID: AB_1720817 |
| β-Actin antibody-HRP | GenScript | Cat# A00730, RRID: AB_914100 |
| Peroxidase-AffiniPure goat anti-mouse IgG (H + L) antibody | Jackson ImmunoResearch Labs | Cat# 115-035-003, RRID: AB_10015289 |
| Peroxidase-AffiniPure goat anti-rabbit IgG (H+L) antibody | Jackson ImmunoResearch Labs | Cat# 111-035-003, RRID: AB_2313567 |
| Peroxidase-AffiniPure goat anti-rat IgG (H+L) antibody | Jackson ImmunoResearch Labs | Cat# 112-035-003, RRID: AB_2338128 |
| Rabbit anti-SREBP1 polyclonal antibody | Novus Biologicals | Cat# NB100-2215; AB_10002406 |
| Mouse anti-dsDNA (HYB331-01) monoclonal antibody | Santa Cruz Biotechnology | Cat# sc-58749; AB_783088 |
| Rabbit anti-HSF1 (H-311) polyclonal antibody | Santa Cruz Biotechnology | Cat# sc-9144, RRID: AB_2120276 |
| Rat anti-HSF1 (10H8) monoclonal antibody | Santa Cruz Biotechnology | Cat# sc-13516, RRID: AB_627751 |
| Mouse anti-biotin Ab-2 (BTN.4) monoclonal antibody | Thermo Fisher Scientific | Cat# MA511251; RRID: AB_10980856 |
| Rabbit anti-AMPKβ1+2 (E.427.6) monoclonal antibody | Thermo Fisher Scientific | Cat# MA515090; RRID: AB_10981956 |
| Rabbit anti-AMPKγ1+2+3 polyclonal antibody | Thermo Fisher Scientific | Cat# PA536314; RRID: AB_2553469 |
| Rabbit anti-GLI1 polyclonal antibody | Thermo Fisher Scientific | Cat# PA5-32206; RRID: AB_2549679 |
| Mouse anti-SREBP1 (2A4) monoclonal antibody | Thermo Fisher Scientific | Cat# MA5-16124, RRID: AB_11152118 |
| Rabbit anti-cholesterol polyclonal antibody | USBiological | Cat# 139711 |
| ImmPRESS® Excel Amplified HRP Polymer Staining Kit (Goat anti-rabbit IgG and horse anti-goat IgG HRP polymers) | Vector Laboratories | Cat# MP-7601, RRID: AB_2336533 |
| Bacterial and Virus Strains | ||
| NEB® 5-α Competent E. coli (High Efficiency) | New England Biolabs | Cat# C2987 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Bafilomycin A1 | Axxora LLC | Cat# LKT-B0025 |
| Chloroquine diphosphate | Axxora LLC | Cat# LKT-C2950 |
| Mant-AMP | Axxora LLC | Cat# JBS-NU-236S |
| Recombinant active human AMPKα1/PRKAA1 proteins | Cell Sciences | Cat# CRA019 |
| AMPKα (23A3) Rabbit monoclonal antibody (Sepharose® Bead Conjugate) | Cell Signaling Technology | Cat# 6707 |
| Recombinant human His-HSF1 proteins | Enzo Life Sciences | Cat# ADI-SPP-900 |
| Recombinant human HSP90β proteins | Enzo Life Sciences | Cat# ALX-201-147-C025 |
| HSF1 peptides (see Table S1: HSF1 peptide sequences) | GenScript | Custom order |
| Puromycin | InvivoGen | Cat# ant-pr-1 |
| 17-DMAG | LC Laboratories | Cat# D-3440 |
| Recombinant human SREBP1 proteins | Lifespan BioSciences, Inc | Cat# LS-G56019 |
| (Z)-4-Hydroxytamoxifen | LKT Laboratories | Cat# H9711 |
| Recombinant bovine kidney PP2A1 proteins | Millipore | Cat# 53-950-8 |
| Lambda phosphatases | New England Biolabs | Cat# P0753S |
| In Vivo-JetPEI® transfection reagent | PolyPlus-transfection | Cat# 201 |
| CellTiter-Blue® cell viability assay kit | Promega | Cat# G8081 |
| Recombinant cholesteroylated human SHH-N proteins | R&D Systems | Cat# 8908-SH-005/CF |
| AMP | Research Product International | Cat# A11240 |
| ATP | Research Product International | Cat# A30030 |
| Dorsomorphin dihydrochloride (Compound C) | Santa Cruz Biotechnology | Cat# sc-361173 |
| A23187 | Sigma-Aldrich | Cat# C7522 |
| Cholesterol-water soluble | Sigma-Aldrich | Cat# C4951 |
| Duolink® In Situ PLA anti-mouse Minus probes | Sigma-Aldrich | Cat# DUO92004 |
| Duolink® In Situ PLA anti-rabbit Plus probes | Sigma-Aldrich | Cat# DUO92002 |
| Duolink® In Situ PLA Detection Reagents Green | Sigma-Aldrich | Cat# DUO92014 |
| Duolink® In Situ PLA Detection Reagents Red | Sigma-Aldrich | Cat# DUO92008 |
| Recombinant active human LKB1/MO25/STRAD proteins | Sigma-Aldrich | Cat# SRP0246 |
| Recombinant human ACC1 proteins | Sigma-Aldrich Corporation | Cat# A6986 |
| Recombinant GST proteins | SignalChem Lifesciences | Cat# G52-30U |
| RNA STAT-60 reagent | Tel-Test, Inc. | Cat# RNA STAT-60 |
| ActinGreen™ 488 ReadyProbes® Reagent | Thermo Fisher Scientific | Cat# R37110 |
| Alfa Aesar™ Okadaic acid | Thermo Fisher Scientific | Cat# NC0314406 |
| Amplex™ Red Cholesterol Assay Kit | Thermo Fisher Scientific | Cat# A12216 |
| Apexbio Technology LLC sanguinarine chloride | Thermo Fisher Scientific | Cat# 50-115-2424 |
| DSP crosslinker | Thermo Fisher Scientific | Cat# PI22585 |
| Gibco™ recombinant human TRAIL proteins | Thermo Fisher Scientific | Cat# PHC1634 |
| Halt™ Protease Inhibitor Cocktail | Thermo Fisher Scientific | Cat# 78430 |
| Mant-ATP | Thermo Fisher Scientific | Cat# M12417 |
| NE-PER™ Nuclear and Cytoplasmic Extraction Reagents | Thermo Fisher Scientific | Cat# PI78835 |
| Nile Red | Thermo Fisher Scientific | Cat# N1142 |
| Oligomycin | Thermo Fisher Scientific | Cat# AAJ61898MA |
| Pierce™ High Sensitivity Streptavidin-HRP | Thermo Fisher Scientific | Cat# 21134 |
| Recombinant human AMPK(A1/B1/G2) proteins | Thermo Fisher Scientific | Cat# PV6238 |
| Selleck Chemical LLC phenformin | Thermo Fisher Scientific | Cat# 50-579-2 |
| Critical Commercial Assays | ||
| EnzyLight® ADP Assay Kit | BioAssay Systems | Cat# EADP-100 |
| PathScan® Phospho-AMPKα (Thr172) Sandwich ELISA Antibody Pair | Cell Signaling Technology | Cat# 7955 |
| Mito-ID® red detection kit | Enzo Life Sciences | Cat# ENZ-51007 |
| Q5® Site-Directed Mutagenesis Kit | New England Biolabs Inc. | Cat# E0552S |
| JetPRIME® transfection reagents | PolyPlus-transfection® SA | Cat# 114 |
| AMP-Glo® Assay Kit | Promega | Cat# V5011 |
| Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit | R&D Systems | Cat# DSHH00 |
| DyNAmo SYBR Green qPCR kit | Thermo Fisher Scientific | Cat# F416XL |
| Thermo Scientific™ Pierce™ Silver Stain Kit for Mass Spectrometry | Thermo Fisher Scientific | Cat# 24600 |
| Verso cDNA Synthesis kit | Thermo Fisher Scientific | Cat# AB1453A |
| Deposited Data | ||
| Raw images of western blots and original image files | Mendeley Data | http://doi.org/10.17632/bpr9h3gpfn.1 |
| Experimental Models: Cell Lines | ||
| A2058 | ATCC | Cat# CRL-11147 |
| HeLa | ATCC | Cat# CCL-2 |
| HEK293T | GE Dharmacon | Cat# HCL4517 |
| Immortalized Rosa26-CreERT2; Hsf1fl/fl MEFs | Su et al., 2016 | N/A |
| Experimental Models: Organisms/Strains | ||
| Hsf1fl/fl mice (C57BL/6J) | Su et al., 2016 | N/A |
| Hsf1−/− mice (129SvJ/BALB/cJ) | Xiao et al., 1999 | N/A |
| Alb-Cre mice (C57BL/6J) | The Jackson Laboratory | IMSR Cat# JAX:003574; RRID:IMSR_JAX:003574 |
| Meox2-Cre mice (C57BL/6J) | The Jackson Laboratory | IMSR Cat# JAX:003755; RRID:IMSR_JAX:003755 |
| NOD.CB17-Prkdcscid/J mice (NOD/SCID) | The Jackson Laboratory | IMSR Cat# JAX:001303; RRID:IMSR_JAX:001303 |
| Oligonucleotides | ||
| qRT-PCR primers (see Table S2: Primer sequences) | IDT | N/A |
| ChIP qPCR primers (see Table S2: Primer sequences) | IDT | N/A |
| Mutagenesis and sequencing primers (see Table S2: Primer sequences) | IDT | N/A |
| The target sequences of siRNAs and shRNAs (see Table S3) | N/A | N/A |
| Recombinant DNA | ||
| pLenti6-LacZ | Su et al., 2016 | N/A |
| pHSE-SEAP | Clontech Laboratories | Cat# 631910 |
| pCMV-Gaussia luciferase | Thermo Fisher Scientific | Cat# 16147 |
| pLenti6-FLAG-HSF11–529 | This paper | N/A |
| pBabe-FLAG-HSF11–323 | This paper | N/A |
| pBabe-FLAG-HSF1324–529 | This paper | N/A |
| pLenti6-FLAG-HSF1324–529 | This paper | N/A |
| pBabe-LacZ | Su et al., 2016 | N/A |
| pBabe-FLAG-HSF11–529 | Gift from Robert Kingston | Addgene #1948 |
| pBabe-FLAG-HSF1S326A | Tang et al., 2015 | N/A |
| pLenti6-FLAG-HSF1S326D | Tang et al., 2015 | N/A |
| pLenti6-FLAG-HSF1Δ49–144,Δ385–480 | This paper | N/A |
| Software and Algorithms | ||
| CDPro | CDPro software | https://sites.bmb.colostate.edu/sreeram/CDPro/ |
| FlowJo | FlowJo, LCC | https://www.flowjo.com/ |
| ImageJ | National Institutes of Health | https://imagej.nih.gov/ij/ |
| Prism 7 and 8 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| Other | ||
| Protein G MagBeads | GenScript | Cat# L00274 |
| HyClone™ bovine growth serum | HyClone Laboratories | Cat# SH30541.03IR |
| Normocin™ | Invivogen | Cat# ant-nr-1 |
| DMEM 4.5g/L Glucose w/ L-Glutamine | Lonza | Cat# 12-604Q |
| MycoAlert™ Mycoplasm Detection kits | Lonza | Cat# LT07-418 |
| Streptavidin Magnetic Beads | New England Biolabs | Cat# S1420S |
| 1-Step™ Ultra TMB-ELISA Substrate Solution | Thermo Fisher Scientific | Cat# 34028 |
| Hoechst 33342 | Thermo Fisher Scientific | Cat# H1399 |
| Normal goat serum | Thermo Fisher Scientific | Cat# OB006001 |
| NovaBright™ Phospha-Light™ EXP Assay Kit for SEAP | Thermo Fisher Scientific | Cat# N10578 |
| Pierce™ Gaussia Luciferase Glow Assay Kit | Thermo Fisher Scientific | Cat# 16160 |
| Pierce™ Streptavidin Coated Plates, Clear, 96-Well | Thermo Fisher Scientific | Cat# 15124 |
| Slide-A-Lyzer MINI Dialysis Devices, 7K MWCO, 0.1ml | Thermo Fisher Scientific | Cat# 69560 |
| SuperSignal West Pico Plus chemiluminescent substrates | Thermo Fisher Scientific | Cat# 34580 |
| SuperSignal™ West Femto Maximum Sensitivity Substrate | Thermo Fisher Scientific | Cat# 34096 |
| TurboFect transfection reagents | Thermo Fisher Scientific | Cat# R0531 |
Contact for Reagent and Resource Sharing
Further information and requests for reagents should be directed to the Lead Contact Chengkai Dai (chengkai.dai@nih.gov).