Figure 3 .

RAN proteins levels are decreased in eIF2α-S51A mutant cells. Protein blots of WT and S51A mutant HEK293T cells co-transfected with CAGG (A) or CCTG (B) expansion vectors and a GFP expressing control plasmid. Protein blots were probed with antibodies against Flag, p-eIF2α, total eIF2α, GFP, p-PKR or GAPDH. Quantification using ImageJ shows the ratio of QAGR or LPAC RAN proteins compared to GAPDH levels (lower panels). Error bars show SD. n = 3. Statistical analyses were performed using the two-tailed t-test, ^*P < 0.05, ^****P < 0.0001.