Figure 5 .

Contribution of eIF2A to RAN translation depends on phosphorylation of eIF2α. Protein blots of WT and S51A mutant HEK293T cells transfected with CAGG (A) or CCTG (B) expansion constructs with or without an eIF2A expressing construct. Protein blots were probed with antibodies against Flag, eIF2A, p-eIF2α and GAPDH. Quantification was done using ImageJ and ratios of RAN proteins to GAPDH are shown (lower panels). Error bars show SD. n = 3. Statistical analyses were performed using the two-tailed t-test, ^*P < 0.05, ^***P < 0.001, ^****P < 0.0001.