Figure 3.
Inhibition effect and inhibition mode of suramin over SARS-CoV-2 3CLpro. (A) 3D representation of the 3CLpro-suramin complex (3CLpro are in ribbon presentation, and suramin is indicated by sticks). 3CLpro monomers are colored in green and blue and suramin in orange. Normalized activity and inhibition of the virus proteases and Lineweaver-Burk plots to determine the inhibition mode are presented. [S] is the substrate concentration; v is the initial reaction rate. (B) Normalized activity and inhibition of SARS-CoV-2 3CLpro under suramin influence. (C) Lineweaver-Burk plot for Suramin inhibition of SARS-CoV-2 3CLpro. Data shown are mean ± SD from 3 independent measurements (n = 3).