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. 2021 Jun 2;16:34. doi: 10.1186/s13024-021-00439-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Mass spectroscopy of the myenteric plexus (MP) from pre-symptomatic (ps)A30P mice compared with wild type (WT) controls. Regulated proteins in the MP of the small intestine (SI, a) and the large intestine (LI, b) were volcano plotted using GraphPad Prism 8 according to their statistical significance. Proteins with significantly altered expression are shown in purple, proteins with non-significantly altered expression in black. Protein-protein interactions obtained from the STRING database are shown for MP samples of the SI (c) and the LI (d). Neurofilament light chain (Nefl), vesicle-associated membrane protein 2 (Vamp2), and calbindin 2 (Calb2) are highlighted with purple circles. (e) In total, 74 proteins were affected in the SI and 147 in the LI. Among these, 13 proteins were changed significantly in both, the SI and the LI. The Venn diagram and the heatmaps were generated using GraphPad Prism 8. Here, only proteins with significantly altered expression were included. Classified functional groups of regulated proteins in the gut (actin and microtubule organization, vesicle transport, calcium binding, ubiquitination, and oxidative stress) are shown in (f). The intensity levels (percentage of normalized volumes) of the protein spots were shown on a heatmap according to their expression levels. Individual rows represent single spots and graduated scale color codes from red (decreased expression levels) to green (increased expression levels) and white (no altered expression levels). Each column represents data from n = 4 independent experiments