Figure 1.

Vapor-diffusion micro-crystallization procedure within the HARE chip. (a) Silicon HARE chip with 6 × 6 compartments containing 24 × 24 features per compartment. The cross section shows the dimensions of the features. (b) A blade is used to spread a minimal amount of crystallization mixture evenly over the surface of the chip and into the features. (c) Six chips can be placed in the crystallization tray to conduct canonical vapor-diffusion crystallization experiments. Sample consumption is minimized to 10 µl crystallization solution (using just 5 µl of protein stock solution) per chip.