Figure 2.

Vapor-diffusion in-chip crystallization of model proteins. In-chip crystallization of the model proteins lysozyme (a–d), proteinase K (e–h) and xylose isomerase (i–l). Top rows: conventional hanging drops are compared with in-chip crystallization using the same crystallization conditions. The inset shows enlarged images of crystals within the features of the HARE chip. The scale bars represent 50 µm. Third row: a representative hit map is shown for each model protein, where blue indicates a recorded diffraction pattern that was successfully indexed by CrystFEL. Bottom row: representative section of the 2F _o − F _c electron-density map (contoured at 2σ) after refinement of data from a single chip.