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. 2021 May 8;11(14):6905–6921. doi: 10.7150/thno.56882

Figure 1.

Figure 1

Epithelial-mesenchymal transition (EMT) of two hepatocellular carcinoma cell lines induced by HGF treatment. A. Workflow of dynamically site-specific glycosylation and global proteome analyses of two hepatocellular carcinoma cell lines during the EMT process. B. The down-regulation of E-cadherin and the up-regulation of N-cadherin were monitored at six time points of HGF treatment (0-72 h) by quantitative real-time PCR (qRT-PCR). Data are presented as mean±SEM of at least triplicates experiments. P values were determined by One-way ANOVA. n.s, no significant; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. C. The EMT process could be classified into three stages based on changes of these two EMT markers. E: epithelial stage (0 h), I: intermediate stage (6 h-12 h), M: mesenchymal stage (24 h-72 h). D. Comparison of intact glycopeptides identified from SMMC-7721 and HepG2 cell lines. E. Distribution of glycan subtypes from intact glycopeptides identified from both cell lines.