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. 2021 May 8;11(14):6905–6921. doi: 10.7150/thno.56882

Figure 6.

Figure 6

Site-specific core fucosylation of FOLR1 is critical for folate uptake. A. qRT-PCR analysis of FOLR1 mRNA expression level in five stable cell lines of site-specific glycosite mutations of FOLR1. The stable cell lines of RFP and FOLR1 wild-type (WT) were used as negative and positive controls, respectively. The data were generated by averaging at least triplicate analyses per condition. GAPDH was used as a control. B. The protein expression of FOLR1 in SMMC-7721 (left) and HepG2 (right) cell lines with glycosite mutations on FOLR1. C, D. The protein expression levels of FUT8, N-cadherin and E-cadherin in FOLR1 mutated cell lines treated by HGF (10 ng/mL) for 24 h. The grayscale value was measured from the western blotting data with Image J. E-G. Immunofluorescence staining of FOLR1 mutated cell lines incubated with FITC-FA for 24 h. 5 fields per replicate, 3 replicates per condition. Scale bar, 30 µm. Relative intensity of red and green immunofluorescent was determined by ImageJ software (n=10). Data are presented as mean ± SEM. P values were determined by One-way ANOVA. n.s, no significance, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.