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. 2021 May 13;11(14):7110–7125. doi: 10.7150/thno.61085

Figure 3.

Figure 3

SRPX2 is elevated in fibroblasts in a TGF-β/SMADs manner. A: Western blot analysis of SRPX2 expression in HPFs following different dose of TGF-β1 induction for 24 h. B: Results for time-course Western blot analysis of SRPX2 expression in HPFs following TGF-β1 (10 ng/ml). C: Results for co-immunostaining of SRPX2 and p-SMAD2/3 in HPFs following TGF-β1 induction for 1h (up), lung sections of pulmonary fibrosis mice (middle), and lung sections from IPF patients (down). The nuclei were stained blue by DAPI, and the images were taken under original magnification ×400. D-E: Western blot (D) and RT-PCR (E) analysis of SRPX2 expression in HPFs pre-treated with SB431542 treatment following TGF-β1 induction. F-G: Western blot (F) and RT-PCR (G) analysis of SRPX2 expression in HPFs pre-treated with SIS3-HCL following TGF-β1 induction. The data are represented as the mean ± SEM of three independent experiments. ***, p < 0.001.