Skip to main content
. 2021 May 12;11(14):6983–7004. doi: 10.7150/thno.57237

Figure 5.

Figure 5

HMG20A coordinates expression of astrocyte derived extracellular factors involved in neuronal survival. (A) Representative immunofluorescent images of mouse spinal cord motoneurons cultured for 24 h in increasing percentage of conditioned media derived from primary astrocytes silenced for HMG20A (siHMG20A) or not (siCT). Live cells are stained with Calcein (green) while dying cells label for Eth-D1 (red). (B) Quantification of motoneuron cell death after growth in the indicated % of astrocyte conditioned media; control siCT astrocytes (black circles) or HMG20A silenced siHMG20A astrocytes (Black triangles). n = 6 biological replicates (independent spinal cord motoneurons primary cultures). * p < 0.05 and ** p < 0.01, unpaired t-test siCT versus siHMG20A. (C) GO Cellular compartment of genes differentially regulated by siHMG20A in astrocytes. % of genes of the indicated categories is represented. p-value of the enrichment is also provided. (D) Vulcano plot showing expression change (logs(FC) and p-value (-log10(p-value)) (siHMG20a versus siCT) of all expressed growth factors encoding genes.