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. 2021 May 8;11(14):6936–6949. doi: 10.7150/thno.58173

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Evaluation of APC activation by the Ncom Gel vaccine in vitro. (A-C) Flow cytometry analysis of the expression of CD80, CD83 and CD86 on BMDCs (n=3, mean ± s.d., **P < 0.01, *** P< 0.001, *** P< 0.0001). (D-E) The secretion of IL-6 and TNF-α in BMDC suspensions detected by ELISA kits (n = 3, mean ± s.d., * P < 0.05, **P < 0.01, *** P< 0.001 and **** P< 0.0001). (F) Schematic illustration of DC activation through multiple stimuli. (G-I) The percentages of CD80⁺ Raw264.7 (A), CD40⁺ Raw264.7 (B) and CD86⁺ Raw264.7 (C) cells in various groups (n=3, mean ± s.d., * P < 0.05, **P < 0.01, *** P< 0.001 and **** P< 0.0001).