Table 2:

Hydrogel formulations for covalently crosslinked alginate. (Adapted with permission from Chaudhuri et al., 2015)

			Syringe 1	Syringe 2			Syringe 3	Final concentrations		Expected mechanical properties
Sample	Avg. MW of alginate (kDa)	Total gel volume (μL)	Volume of 3% wt/vol alginate (μL)	AAD/HOBT concentration	Volume of AAD/HOBT solution (μL)	Volume of MES buffer (μL)	Volume of EDC solution (μL)	AAD crosslinker concentration (mM)	Alginate concentration (wt/vol, %)	Initial elastic modulus (kPa)	Stress relaxation
Covalently crosslinked, high stiffness	280	1200	800	1X AAD	120	160	120	5.74	2	9.7	None
Covalently crosslinked, inter. Stiffness	280	1200	800	0.2X AAD	120	160	120	1.15	2	3.4	None
Covalently crosslinked, low stiffness	280	1200	800	0.2X AAD	60	220	120	0.57	2	1.5	None