Table 2:
Syringe 1 | Syringe 2 | Syringe 3 | Final concentrations | Expected mechanical properties | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Sample | Avg. MW of alginate (kDa) | Total gel volume (μL) | Volume of 3% wt/vol alginate (μL) | AAD/HOBT concentration | Volume of AAD/HOBT solution (μL) | Volume of MES buffer (μL) | Volume of EDC solution (μL) | AAD crosslinker concentration (mM) | Alginate concentration (wt/vol, %) | Initial elastic modulus (kPa) | Stress relaxation |
Covalently crosslinked, high stiffness | 280 | 1200 | 800 | 1X AAD | 120 | 160 | 120 | 5.74 | 2 | 9.7 | None |
Covalently crosslinked, inter. Stiffness | 280 | 1200 | 800 | 0.2X AAD | 120 | 160 | 120 | 1.15 | 2 | 3.4 | None |
Covalently crosslinked, low stiffness | 280 | 1200 | 800 | 0.2X AAD | 60 | 220 | 120 | 0.57 | 2 | 1.5 | None |