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Formulations for coupling 5kDa and 20kDa PEG to alginate with varying degrees of substitution. Note that the molecular weight of all alginate samples here is 280kDa.
| Sample | Alginate (mg) | PEG (mg) | Sulfo-NHS (mg) | EDC (mg) |
|---|---|---|---|---|
| 5kDa-DS16 | 200 | 95.2 | 43.8 | 77.5 |
| 5kDa-DS32 | 200 | 190.5 | 87.7 | 154.9 |
| 20kDa-DS4 | 200 | 95.2 | 11 | 19.4 |
| 20kDa-DS8 | 200 | 190.5 | 21.9 | 38.7 |
(Adapted with permission from Nam, Stowers, et al., 2019).
