FIGURE 2.

Comparative analysis of the electrophysiological features of native yVDAC1 and yVDAC2 at the Planar Lipid Bilayer. (A) Analysis of conductance after yVDACs reconstitution into the artificial membrane. Experiments were performed with an applied constant voltage of +10 mV in 1 M KCl solution. (B) Analysis of the voltage-dependence of yVDACs isoforms. Experiments were performed by gradually increasing the voltage from 0 to ±100 mV. Data are expressed as mean of the relative conductance ± SEM. The relative conductance was calculated as the G/G₀, where G is the conductance at the given voltage while G₀ is the conductance values calculated at 0 mV applied. (C) Analysis of current-voltage performed in a 10-fold gradient 1/0.1 M cis/trans KCl in a voltage ramp with amplitude ± 60 mV. Values of reversal potential were used to estimate the permeability ratio of cations (P_K) over anions (P_Cl) by using the Goldman-Hodgkin-Katz equation in the three states.