Table 1.
Primary and secondary antibody used in Western blot
| Classification | Items | Source | Titer | Company |
|---|---|---|---|---|
| Primary antibody | PGC-1α | Anti-mouse | 1:1,000 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
| FNDC5 | Anti-rabbit | 1:1,000 | Abcam, Cambridge, UK | |
| AMPK, p-AMPK | Anti-rabbit | 1:1,000 | Cell Signaling Technology, Inc., Danvers, MS, USA | |
| Secondary antibody | HRP-conjugated IgG | Mouse | 1:2,000 | Vector Laboratories, Burlingame, CA, USA |
| Rabbit |
PGC-1α, peroxisome proliferator-activated receptor gamma coactivator-1α; FNDC5, fibronectin type III domain-containing protein 5; p-AMPK, phosphorylation adenosine monophosphate-activated protein kinase; HRP, horseradish peroxidase.