Figure 6.

RASA4 gene expression is required for T. denticola-mediated actin stress fiber dysfunction. (A) Quantitative RT-PCR analysis of RASA4 relative fold change in PDL cells infected with scramble shRNA or RASA4 shRNA. (B) Representative confocal images and (C) relative optical intensity of stress fiber intensity in scramble shRNA, RASA4 shRNA and RASA4shRNA cells challenged cells with T. denticola (50 MOI) for 24 h and stained with SiR-Actin dye (1:5000). Data represent mean ± SD from three independent experiments. Data in panel (A) was analyzed using an Unpaired t test. Data in panel (C) was analyzed using One-way ANOVA Tukey’s Multiple comparisons test. * = p<0.05; N.S., Not significant.