Cytotoxicity and hemolytic activity of natural and synthetic glycolipids (1–6).
| Glycolipida | Cytotoxicity | Hemolysis | ||
|---|---|---|---|---|
| (IC50 in μM)b | (HC50 in μM)c | |||
| A549 | DLD-1 | WS1 | Erythrocytes | |
| 1 | 50 ± 7 | 58 ± 2 | 52 ± 4 | 12.3 ± 0.3 |
| 2 | >200d | >200d | >200d | >200d |
| 3 | >200d | >200d | >200d | 65 ± 6 |
| 4 | 56 ± 2 | 70 ± 10 | 57 ± 1 | 12.0 ± 0.7 |
| 5β | 103 ± 7 | 110 ± 2 | 105 ± 5 | 14 ± 2 |
| 5α | >200d | >200d | >200d | >200d |
| 6β | 112 ± 2 | 112 ± 3 | 110 ± 1 | 12.3 ± 0.5 |
| 6α | >200d | >200d | >200d | >200d |
a
Synthetic samples.
b
Half maximal inhibitory concentration measured via the resazurin assay. Etoposide was used as a positive control80 showing IC50 values of 1.2, 27, and 34 μM against A549, DLD-1, and WS1 cell lines, respectively.
c
Half maximal inhibitory concentration measured on sheep blood erythrocytes. Triton X-100 was used as a positive control showing an HC50 value of 52 ± 2 μM.
d
No inhibition or activity at the maximum tested concentration (IC50 or HC50 > 200 μM).