Fig. 3. Liquid droplet characteristics of wt p53C (100 μM) at various temperatures and times. (a) and (b) Phase separation (PS) of p53C in the presence of a molecular crowding agent (15% w/v PEG-4000). No phase separation is observed in the absence of PEG or in the absence of p53 protein. (c) Diagram of p53C LLPS temperature (4, 15 and 37 °C) versus time (15, 30 and 60 min), showing the morphology of droplets, spheroids and aggregates under various conditions.
